Analysis Note

Positive ControlConditioned media from dexamethasone-treated (1 µM) HT-1080 cells

Application

Immunoblotting (1 µg/ml)r>Immunoprecipitation (see application references)r>Immunocytochemistry (see application referencesr>Neutralization Studies (see application references)

General description

This Anti-PAI-1 (Ab-1) Mouse mAb (2) is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of PAI-1 (Ab-1).

Purified mouse monoclonal antibody (see application references). Recognizes both active and latent forms of the ~50 kDa PAI-1 protein.

Recognizes both active and latent forms of the ~50 kDa PAI-1 protein in conditioned media from dexamethasone-treated HT-1080 cells. Sold under license of U.S. Patent 5,422,245.

Immunogen

partially purified PAI-1 from conditioned medium of dexamethasone-treated HT1080 human cells

Human

Legal Information

Sold under license of U.S. Patent 5,422,245.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Janicke, F., et al. 1994. Cancer Research.54, 2527.Lawrence, D. A., et al. 1994. J. Biol. Chem.269, 15223.Nagayama, M., et al. 1994. Cancer73, 1398.Petersen, H., et al. 1994. Cancer Res.54, 120.Keijer, J., et al. 1991. Blood78, 401.Blasi, F. and Verde, P., 1990. in Seminars in Cancer Biol.Vol. 1, 153.Declerck, P. J. and Collen, D., 1990. Thrombosis Res.Supplement X 3.Nielsen, L. S., et al. 1986. Thrombosis and Haemostasis.55, 206.

Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Physical form

Lyophilized from a volatile buffer, 100 µg BSA.

Reconstitution

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.

Warning

Toxicity: Standard Handling (A)